23
DOI: https://doi.org/10.1186/s43141-023-00519-4
English
eng
Rafanomezantsoa, Pelias
Contributor
<div id="abs0005" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0010" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Background</h3><div class="u-margin-s-bottom" id="sp0045" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;">Over the years, excessive use of chemical pesticides to control <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/plant-pathogens" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">plant pathogens</a><span style="margin: 0px; padding: 0px;"> has caused environmental problems. Therefore, biological solutions such as the use of microorganisms with antimicrobial capacity become indispensable. To inhibit the growth of plant pathogens, <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/biological-control-agents" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">biological control agents</a> use different mechanisms, including the production of </span></span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/hydrolase" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">hydrolytic enzymes</a><span style="margin: 0px; padding: 0px;">. In this study, the production of <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/amylase" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">amylase</a><span style="margin: 0px; padding: 0px;">, an enzyme important for the prevention and <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/plant-disease-management" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">control of plant diseases</a>, by a biological control agent </span></span></span><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/bacilli" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Bacillus</a></span><em style="margin: 0px; padding: 0px;"> halotolerans</em></span><span style="margin: 0px; padding: 0px;"> RFP74 was optimized using <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/response-surface-methodology" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">response surface methodology</a>.</span></div></div><div id="abs0010" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0015" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Results</h3><div class="u-margin-s-bottom" id="sp0050" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><em style="margin: 0px; padding: 0px;">Bacillus halotolerans</em><span style="margin: 0px; padding: 0px;"> RFP74 inhibited the growth of various <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/phytopathogen" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">phytopathogens</a> including </span><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/alternaria" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Alternaria</a></span></span> and <span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/bipolaris" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Bipolaris</a></span></span><span style="margin: 0px; padding: 0px;"> with an inhibition rate of more than 60%. In addition, it also demonstrated an essential production of <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/amylase" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">amylase</a>. Based on previous studies of amylase production in </span><em style="margin: 0px; padding: 0px;">Bacillus</em><span style="margin: 0px; padding: 0px;">, three parameters were considered significant: initial pH of the medium, <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/incubation-time" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">incubation time</a>, and temperature. Using the central composite design with Design Expert software, the optimized amylase production for </span><em style="margin: 0px; padding: 0px;">B</em>.<em style="margin: 0px; padding: 0px;">halotolerans</em><span style="margin: 0px; padding: 0px;"> RFP74 is at a temperature of 37 °C, <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/incubation-time" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">incubation time</a> 51 h and pH 6.</span></div></div><div id="abs0015" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0020" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Conclusion</h3><div class="u-margin-s-bottom" id="sp0055" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;">The biological control agent <em style="margin: 0px; padding: 0px;">B</em>.<em style="margin: 0px; padding: 0px;">halotolerans</em> RFP74 inhibited the growth of <span style="margin: 0px; padding: 0px;"><em style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/alternaria" title="Learn more about Alternaria from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Alternaria</a></em></span> and <span style="margin: 0px; padding: 0px;"><em style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/bipolaris" title="Learn more about Bipolaris from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Bipolaris</a></em></span><span style="margin: 0px; padding: 0px;">, demonstrating its broad spectrum of activity. Knowledge of the optimal condition required for the production of <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/hydrolase" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">hydrolytic enzymes</a> such as amylase provides information on the most effective application of this biological control agent.</span></div></div>
Elsevier
December 2023
pdf
Journal of Genetic Engineering and Biotechnology
Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology
mixed material
Elsevier