23
DOI: https://doi.org/10.1186/s43141-023-00523-8
English
eng
Vrundha, Chandran Padikkal Krishna
Contributor
<p><ul id="issue-navigation" class="issue-navigation u-margin-s-bottom u-bg-grey1" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px; list-style: none; background-color: rgb(245, 245, 245) !important; overflow: hidden; font-size: 16px; line-height: 24px; color: rgb(31, 31, 31); font-family: ElsevierSans, Arial, Helvetica, Roboto, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><li class="previous move-left u-padding-s-ver u-padding-s-left" style="box-sizing: border-box; margin: 0px; padding-top: 16px !important; padding-right: 0px; padding-bottom: 16px !important; padding-left: 16px !important; float: left; left: 0px;"><a class="button-alternative button-alternative-tertiary u-display-flex button-alternative-icon-left" href="https://www.sciencedirect.com/science/article/pii/S1687157X2301051X" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; --sd-ui-button-alt-border-bottom-colour: transparent; --sd-ui-button-alt-border-bottom-colour-hover: #eb6500; --sd-ui-button-alt-border-bottom-width: 2px; --sd-ui-button-alt-colour: #1f1f1f; --sd-ui-button-alt-colour-focus: #eb6500; --sd-ui-button-alt-cursor: pointer; --sd-ui-button-alt-font-size: 1em; --sd-ui-button-alt-gap: calc(0.5em - 2px); --sd-ui-button-alt-min-width: 5.5rem; --sd-ui-button-alt-icon-background-colour: transparent; --sd-ui-button-alt-icon-background-colour-hover: transparent; --sd-ui-button-alt-icon-border-colour: #1f1f1f; --sd-ui-button-alt-icon-border-colour-hover: #eb6500; --sd-ui-button-alt-icon-fill: #1f1f1f; --sd-ui-button-alt-icon-fill-hover: #1f1f1f; --sd-ui-button-alt-icon-padding: 0.5rem; --sd-ui-button-alt-icon-size: 2.5rem; --sd-ui-button-alt-icon-external-link-size: 0.625rem; -webkit-box-align: baseline; align-items: baseline; border: none; color: rgb(31, 31, 31); cursor: pointer; display: flex !important; font-family: inherit; font-size: 16px; gap: 6px; line-height: 24px; min-width: 88px; text-decoration: none; user-select: text;"><svg focusable="false" viewBox="0 0 54 128" height="20" class="icon icon-navigate-left"><path d="M1 61l45-45 7 7-38 38 38 38-7 7z"></path></svg></a></li></ul></p><div class="Abstracts u-font-serif" id="abstracts" style="box-sizing: border-box; margin: 0px; padding: 0px; --sd-ui-line-height: calc(1em + 10px); line-height: 26px; font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif !important; color: rgb(31, 31, 31); font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><div class="abstract author" id="ab0005" style="box-sizing: border-box; margin: 0px 0px 8px; padding: 0px;"><div id="abs0005" style="box-sizing: border-box; margin: 0px; padding: 0px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0010" style="box-sizing: border-box; margin-top: 24px !important; margin-right: 0px; margin-bottom: 8px !important; margin-left: 0px; padding: 0px; font-weight: normal !important; font-size: 20px !important; line-height: 30px !important;">Background</h3><div class="u-margin-s-bottom" id="sp0080" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px;"><em style="box-sizing: border-box; margin: 0px; padding: 0px;">Thottea siliquosa</em><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span>(Lamk.) Ding Hou., an important medicinal shrub, is widely used in both ayurvedic and indigenous systems of medicine. Root being the most useful part, the plant is constantly uprooted and thus puts pressure on the natural population. Until date, no<span> </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/micropropagation" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">micropropagation</a><span> </span>study is available in this plant. The objective of the study is to develop an efficient in vitro propagation protocol and assessment of clonal fidelity of<span> </span></span><em style="box-sizing: border-box; margin: 0px; padding: 0px;">T. siliquosa</em>.</div></div><div id="abs0010" style="box-sizing: border-box; margin: 0px; padding: 0px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0015" style="box-sizing: border-box; margin-top: 24px !important; margin-right: 0px; margin-bottom: 8px !important; margin-left: 0px; padding: 0px; font-weight: normal !important; font-size: 20px !important; line-height: 30px !important;">Results</h3><div class="u-margin-s-bottom" id="sp0085" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">Media browning was a serious issue during micropropagation, and the addition of 40.0 mg/L<span> </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/ascorbic-acid" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">ascorbic acid</a><span> </span>reduced the media browning. For direct shoot regeneration, the optimum response (92% frequency with 20.9 shoots per explant) was obtained when 7-day-old<span> </span></span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/cotyledon" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">cotyledons</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span>were cultured on WPM supplemented with 1.0 mg/L<span> </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/thidiazuron" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">thidiazuron</a><span> </span>and 0.25 mg/L α-naphthalene acetic acid. The cultures were transferred to WPM augmented with 0.4 mg/L thidiazuron for shoot elongation and growth. On this medium, 100% of cultures responded with a mean number of 27.6 shoots. For callus induction, MS medium with 1.0 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L N</span></span><sub style="box-sizing: border-box; margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">6</sub><span style="box-sizing: border-box; margin: 0px; padding: 0px;">-benzylaminopurin was used. Shoot<span> </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/organogenesis" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">organogenesis</a><span> </span>was initiated on the same medium, and calli with minute shoots were transferred to MS medium fortified with 0.5 mg/L N</span><sub style="box-sizing: border-box; margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">6</sub><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">-benzylaminopurin and 0.25 mg/L α-naphthalene acetic acid for highest shoot regeneration (100% cultures responded with a mean number of 26.5 shoots per explant). Maximum rooting frequency (82%) and number (20.8) were obtained on half-strength MS medium with 1.0 mg/L indole-3-butyric acid. The rooted plants were acclimatized and transferred to the field. The<span> </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/high-performance-thin-layer-chromatography" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">HPTLC</a><span> </span>and<span> </span></span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/start-codon" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">SCoT</a><span> </span>analysis revealed the<span> </span></span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/phytochemical" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">phytochemical</a><span> </span>and clonal similarity between the in vitro propagated plants and mother plant.</span></div></div><div id="abs0015" style="box-sizing: border-box; margin: 0px; padding: 0px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0020" style="box-sizing: border-box; margin-top: 24px !important; margin-right: 0px; margin-bottom: 8px !important; margin-left: 0px; padding: 0px; font-weight: normal !important; font-size: 20px !important; line-height: 30px !important;">Conclusions</h3><div class="u-margin-s-bottom" id="sp0090" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">In this study, it is confirmed that cotyledon is an excellent<span> </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/explant" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">explant</a><span> </span>for direct and indirect shoot organogenesis in<span> </span></span><em style="box-sizing: border-box; margin: 0px; padding: 0px;">T. siliquosa</em><span style="box-sizing: border-box; margin: 0px; padding: 0px;">. For direct shoot induction WPM and indirect organogenesis, MS medium was found to give better response. The true-to-type nature of in vitro-derived plants were confirmed by<span> </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/phytochemical" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">phytochemical</a><span> </span>and SCoT analysis. The protocol described here could be used for the large-scale propagation of elite clones of<span> </span></span><em style="box-sizing: border-box; margin: 0px; padding: 0px;">T. siliquosa</em>.</div></div></div></div>
Elsevier
December 2023
pdf
Journal of Genetic Engineering and Biotechnology
Control of media browning during micropropagation and assessment of biochemical and clonal fidelity of in vitro-derived and mother plants in Thottea siliquosa (Lamk.) Ding Hou., an important ethnomedicinal shrub
mixed material
Elsevier