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Paghadar, C.
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<p><div id="reading-assistant-main-body-section" style="box-sizing: border-box; margin: 0px; padding: 0px;"></div><ul id="issue-navigation" class="issue-navigation u-margin-s-bottom u-bg-grey1" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px; list-style: none; background-color: rgb(245, 245, 245) !important; overflow: hidden; font-size: 16px; line-height: 24px;"><li class="previous move-left u-padding-s-ver u-padding-s-left" style="box-sizing: border-box; margin: 0px; padding-top: 16px !important; padding-right: 0px; padding-bottom: 16px !important; padding-left: 16px !important; float: left; left: 0px;"><a class="button-alternative button-alternative-tertiary u-display-flex button-alternative-icon-left" href="https://www.sciencedirect.com/science/article/pii/S1878535219301492" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; --sd-ui-button-alt-border-bottom-colour: transparent; --sd-ui-button-alt-border-bottom-colour-hover: #eb6500; --sd-ui-button-alt-border-bottom-width: 2px; --sd-ui-button-alt-colour: #1f1f1f; --sd-ui-button-alt-colour-focus: #eb6500; --sd-ui-button-alt-cursor: pointer; --sd-ui-button-alt-font-size: 1em; --sd-ui-button-alt-gap: calc(0.5em - 2px); --sd-ui-button-alt-min-width: 5.5rem; --sd-ui-button-alt-icon-background-colour: transparent; --sd-ui-button-alt-icon-background-colour-hover: transparent; --sd-ui-button-alt-icon-border-colour: #1f1f1f; --sd-ui-button-alt-icon-border-colour-hover: #eb6500; --sd-ui-button-alt-icon-fill: #1f1f1f; --sd-ui-button-alt-icon-fill-hover: #1f1f1f; --sd-ui-button-alt-icon-padding: 0.5rem; --sd-ui-button-alt-icon-size: 2.5rem; --sd-ui-button-alt-icon-external-link-size: 0.625rem; -webkit-box-align: baseline; align-items: baseline; border: none; color: var(--sd-ui-button-alt-colour); cursor: var(--sd-ui-button-alt-cursor); display: flex !important; font-family: inherit; font-size: var(--sd-ui-button-alt-font-size); gap: var(--sd-ui-button-alt-gap); line-height: var(--sd-ui-line-height); min-width: var(--sd-ui-button-alt-min-width); text-decoration: none; user-select: text;"><svg focusable="false" viewBox="0 0 54 128" height="20" class="icon icon-navigate-left" style="color: rgb(31, 31, 31); font-family: ElsevierSans, Arial, Helvetica, Roboto, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><path d="M1 61l45-45 7 7-38 38 38 38-7 7z"></path></svg></a></li></ul></p><div class="Abstracts u-font-serif" id="abstracts" style="box-sizing: border-box; margin: 0px; padding: 0px; --sd-ui-line-height: calc(1em + 10px); line-height: var(--sd-ui-line-height); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif !important;"><div class="abstract author" id="ab005" lang="en" style="box-sizing: border-box; margin: 0px 0px 8px; padding: 0px;"><div id="as005" style="box-sizing: border-box; margin: 0px; padding: 0px;"><div class="u-margin-s-bottom" id="sp0130" style="box-sizing: border-box; margin-top: 0px; margin-right: 0px; margin-bottom: 16px !important; margin-left: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">Two simple, specific, sensitive, accurate and precise stability indicating methods were described for<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/quantitative-technique" title="Learn more about quantitative determination from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">quantitative determination</a><span> </span>of the<span> </span></span><a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/anthelmintic" title="Learn more about anthelmintics drug from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">anthelmintics drug</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span><a href="https://www.sciencedirect.com/topics/chemistry/niclosamide" title="Learn more about Niclosamide from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Niclosamide</a>. The first method was high performance liquid chromatographic with the use of a reversed phase hibar</span></span><sup style="box-sizing: border-box; margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">R</sup><span> </span>C-18 column (250 mm × 4.66 mm, 5 <span style="box-sizing: border-box; margin: 0px; padding: 0px;">μm) and<span> </span><a href="https://www.sciencedirect.com/topics/chemical-engineering/mobile-phase-composition" title="Learn more about mobile phase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">mobile phase</a><span> </span>of methanol: 1</span> <span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">mM<span> </span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/ammonium-phosphate" title="Learn more about ammonium phosphate from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">ammonium phosphate</a><span> </span>buffer (85:15 v/v) at a<span> </span></span><a href="https://www.sciencedirect.com/topics/chemistry/flow-kinetics" title="Learn more about flow rate from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">flow rate</a><span> </span>of 1.2</span> <span style="box-sizing: border-box; margin: 0px; padding: 0px;">mL/min. The<span> </span><a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/retention-time" title="Learn more about retention time from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">retention time</a><span> </span>of drug was found to be 6.45</span> ± 0.02 <span style="box-sizing: border-box; margin: 0px; padding: 0px;">min. Quantification of drug was achieved with<span> </span><a href="https://www.sciencedirect.com/topics/engineering/diode-array" title="Learn more about diode array from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">diode array</a><span> </span>detection (DAD) at 332</span> <span style="box-sizing: border-box; margin: 0px; padding: 0px;">nm. Linear<span> </span><a href="https://www.sciencedirect.com/topics/engineering/calibration-curve" title="Learn more about calibration curve from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">calibration curve</a><span> </span>was obtained in concentration range 0.01–100</span> μg/mL with<span> </span><em style="box-sizing: border-box; margin: 0px; padding: 0px;">r</em><sup style="box-sizing: border-box; margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">2</sup><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span>value of 0.999. The limit of detection and<span> </span><a href="https://www.sciencedirect.com/topics/engineering/limit-of-quantification" title="Learn more about limit of quantification from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">limit of quantification</a><span> </span>were found to be 0.048</span> μg/mL and 0.01 <span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">μg/ml respectively. The second method involved a high performance thin layer liquid chromatographic.<span> </span><a href="https://www.sciencedirect.com/topics/engineering/chromatography" title="Learn more about Chromatographic separation from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Chromatographic separation</a><span> </span>was carried out with precoated<span> </span></span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/silica-gel" title="Learn more about silica gel from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">silica gel</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span>G60 F254<span> </span><a href="https://www.sciencedirect.com/topics/chemistry/aluminum" title="Learn more about aluminum from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">aluminum</a><span> </span>sheets using toluene:ethyl acetate (7:3% v/v) as a<span> </span></span></span><a href="https://www.sciencedirect.com/topics/chemistry/mobile-phase-composition" title="Learn more about mobile phase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">mobile phase</a>. Linearity of proposed method was found to be 200–700</span> ng/band at 332 nm with retention factor of 0.59 and<span> </span><em style="box-sizing: border-box; margin: 0px; padding: 0px;">r</em><sup style="box-sizing: border-box; margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; top: -0.5em;">2</sup><span> </span>value of 0.998. The limit of detection and limit of quantification were found to be 36.21 ng/band and 109.7 <span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;">ng/band respectively. Both the developed methods were successfully validated as per International Conference on Harmonization guideline (ICH).<span> </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/niclosamide" title="Learn more about Niclosamide from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Niclosamide</a><span> </span>was subjected to different stress conditions. The degraded product peaks were well resolved from the<span> </span></span><a href="https://www.sciencedirect.com/topics/chemistry/purity" title="Learn more about pure from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">pure</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"><span> </span>drug peak with significant difference in their<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/retention-time" title="Learn more about retention time from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="box-sizing: border-box; margin: 0px; padding: 0px; background-color: transparent; word-break: break-word; text-decoration: underline 1px rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">retention time</a>. Stress samples were successfully assayed by developed high performance liquid chromatographic and high performance thin layer liquid chromatographic method. Statistically analysis proves that there were no statistical significant differences between two developed methods.</span></span></div></div></div></div>
Elsevier Ltd.
December 2019
pdf
Arabian Journal of Chemistry
Development and validation of stability indicating RP-HPLC and HPTLC for determination of Niclosamide in bulk and in synthetic mixture
mixed material
Elsevier Ltd.