23
DOI: https://doi.org/10.1186/s43141-021-00211-5
English
eng
Borah, Rajib
Contributor
<div id="abs0005" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0010" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Background</h3><div class="u-margin-s-bottom" id="sp0100" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><span style="margin: 0px; padding: 0px;"><em style="margin: 0px; padding: 0px;"><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/illicium" title="Learn more about Illicium from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">Illicium</a></em><em style="margin: 0px; padding: 0px;"> griffithii</em></span><span style="margin: 0px; padding: 0px;"> is an aromatic medicinal tree species that has been listed in the IUCN Red List as an <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/endangered-species" title="Learn more about endangered species from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">endangered species</a>. Dried seed pods of </span><em style="margin: 0px; padding: 0px;">I. griffithii</em><span style="margin: 0px; padding: 0px;"> have a good market potential in the spices and pharmaceutical industries. Fruits are the potential source of shikimic acid and used for the production of <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/oseltamivir" title="Learn more about oseltamivir from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">oseltamivir</a><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;"> (a drug against bird flu). However, in recent years, unscientific harvesting and rampant exploitation of the species has caused a negative and adverse effect on its natural population. Proper knowledge of <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/genetic-divergence" title="Learn more about genetic diversity from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">genetic diversity</a><span style="margin: 0px; padding: 0px;"> and population structure is crucial to understand the population dynamics, adaptation, and evolutionary pattern of a particular species for conservation. It was from this view point that the present study was undertaken so as to compare the various types of DNA-based molecular markers namely <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/rapd" title="Learn more about RAPD from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">RAPD</a>, ISSR, DAMD, and </span></span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/start-codon" title="Learn more about SCoT from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">SCoT</a> by their efficiency and SPAR approach to evaluate the genetic diversity of </span></span><em style="margin: 0px; padding: 0px;">I. griffithii</em><span style="margin: 0px; padding: 0px;"> as well as to analyze <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/population-genetics" title="Learn more about population genetic from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">population genetic</a> structure for conservation purpose.</span></div></div><div id="abs0010" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0015" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Result</h3><div class="u-margin-s-bottom" id="sp0105" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;">A total of 250 discernible bands were generated with 246 bands (98.40 %) being polymorphic in nature. All the primers in combination gave a mean polymorphic information content (PIC) of 0.81 and Rp value (resolving power) of 4.32. Nei’s, Gst, and AMOVA analysis showed similar values of genetic differentiation among populations (Gst = 0.396, F<span style="margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em;">ST</span> = 0.30, respectively), revealing a low level of genetic differentiation among the eight sampled populations. <em style="margin: 0px; padding: 0px;">I. griffithii</em> with an estimated gene flow value of Nm = 0.761 was significantly low among populations. Clustering pattern obtained with Bayesian structure and PCoA diagram revealed that intermixing of genetic material across populations is only possible when the populations lie close to each other. This is further validated with UPGMA clustering method where a positive correlation of genetic variability with geographical distance among closely related populations could be clearly seen.</div></div><div id="abs0015" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0020" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Conclusion</h3><div class="u-margin-s-bottom" id="sp0110" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><span style="margin: 0px; padding: 0px;">The result aids in the identification, collection, and preservation of diverse <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/germplasm" title="Learn more about germplasm from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">germplasm</a> of </span><em style="margin: 0px; padding: 0px;">I. griffithii</em> from Arunachal Pradesh and Meghalaya of Northeast India. This would further help in understanding the population structure and genetic diversity among other <em style="margin: 0px; padding: 0px;">Illicium</em> species in order to formulate effective conservation strategies for the improvement of this endangered taxa.</div></div>
Elsevier
December 2021
pdf
Journal of Genetic Engineering and Biotechnology
Genetic diversity and population structure assessment using molecular markers and SPAR approach in Illicium griffithii, a medicinally important endangered species of Northeast India
mixed material
Elsevier