23
DOI: https://doi.org/10.1186/s43141-022-00398-1
English
eng
Haq, Shamshadul
Contributor
<div id="abs0005" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0010" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Background</h3><div class="u-margin-s-bottom" id="sp0080" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><em style="margin: 0px; padding: 0px;">Capsicum</em><span style="margin: 0px; padding: 0px;"> or chilli is an important crop in India which exhibits immense structural and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/genetics" title="Learn more about genetic from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">genetic</a> variations reflecting their intra- and inter-specific relationships. The aim of this study was to establish relationships amongst 54 </span><em style="margin: 0px; padding: 0px;">Capsicum</em><span style="margin: 0px; padding: 0px;"> accessions through analysis of genetic and population structure using <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/inter-simple-sequence-repeat" title="Learn more about ISSR from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">ISSR</a> markers.</span></div></div><div id="abs0010" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0015" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Results</h3><div class="u-margin-s-bottom" id="sp0085" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;"><span style="margin: 0px; padding: 0px;"><span style="margin: 0px; padding: 0px;">Out of 19, successful <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/gene-amplification" title="Learn more about DNA amplifications from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">DNA amplifications</a> were shown by 7 ISSR primers and a total of 80 bands were identified ranging between 8 and 14 with an average of 11.43 bands/primer. A significant degree of polymorphic information content (PIC), discriminating power (DP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) were identified as 0.39, 0.70, 6.40, 5.88, and 2.30, respectively, using ISSR markers in </span><a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/cayenne-pepper" title="Learn more about chillies from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">chillies</a>. The cross-transferability ranged from 8.0 to 72.15% with an average of 52.63% among chillies. Amongst genetic information, grand mean values were 0.264, 0.180, 0.376, 0.296, and 0.180, which correspond to Shannon’s information index (</span><em style="margin: 0px; padding: 0px;">I</em><span style="margin: 0px; padding: 0px;">), expected <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/heterozygosity" title="Learn more about heterozygosity from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">heterozygosity</a> (</span><em style="margin: 0px; padding: 0px;">He</em>), <em style="margin: 0px; padding: 0px;">Nei</em>’<em style="margin: 0px; padding: 0px;">s</em> gene diversity, total diversity among species (<em style="margin: 0px; padding: 0px;">Ht</em>), diversity within species (<em style="margin: 0px; padding: 0px;">Hs</em>), respectively. Further, the coefficients of gene differentiation (<em style="margin: 0px; padding: 0px;">Gst</em>) and gene flow (<em style="margin: 0px; padding: 0px;">Nm</em>) were 0.393 and 0.773, representing higher genetic variation among the population which was confirmed by analysis of molecular variance (AMOVA).</div></div><div id="abs0015" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><h3 class="u-h4 u-margin-m-top u-margin-xs-bottom" id="sect0020" style="margin-right: 0px; margin-left: 0px; padding: 0px; margin-top: 24px !important; margin-bottom: 8px !important; font-size: 20px !important; line-height: 30px !important;">Conclusion</h3><div class="u-margin-s-bottom" id="sp0090" style="margin-top: 0px; margin-right: 0px; margin-left: 0px; padding: 0px; margin-bottom: 16px !important;">ISSR markers represented a potent system for the estimation of relationships or variation studies and generated information useful for planning <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/crop-management" title="Learn more about crop management from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; background-color: rgba(0, 0, 0, 0); word-break: break-word; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(31, 31, 31); color: rgb(31, 31, 31); text-underline-offset: 1px;">crop management</a> and improvement strategies in chilli breeding.</div></div>
Elsevier
December 2022
pdf
Journal of Genetic Engineering and Biotechnology
Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights
mixed material
Elsevier