Optimization of amylase production by the biological control agent Bacillus halotolerans RFP74 using response surface methodology

Background

Over the years, excessive use of chemical pesticides to control plant pathogens has caused environmental problems. Therefore, biological solutions such as the use of microorganisms with antimicrobial capacity become indispensable. To inhibit the growth of plant pathogens, biological control agents use different mechanisms, including the production of hydrolytic enzymes. In this study, the production of amylase, an enzyme important for the prevention and control of plant diseases, by a biological control agent Bacillus halotolerans RFP74 was optimized using response surface methodology.

Results

Bacillus halotolerans RFP74 inhibited the growth of various phytopathogens including Alternaria and Bipolaris with an inhibition rate of more than 60%. In addition, it also demonstrated an essential production of amylase. Based on previous studies of amylase production in Bacillus, three parameters were considered significant: initial pH of the medium, incubation time, and temperature. Using the central composite design with Design Expert software, the optimized amylase production for B.halotolerans RFP74 is at a temperature of 37 °C, incubation time 51 h and pH 6.

Conclusion

The biological control agent B.halotolerans RFP74 inhibited the growth of Alternaria and Bipolaris, demonstrating its broad spectrum of activity. Knowledge of the optimal condition required for the production of hydrolytic enzymes such as amylase provides information on the most effective application of this biological control agent.

Loại tài liệu:

Article - Bài báo

Tác giả:

Rafanomezantsoa, Pelias

Đề mục:

Journal of Genetic Engineering and Biotechnology

Nhà xuất bản:

Elsevier

Ngày xuất bản:

December 2023

Số trang/ tờ:

Định dạng:

pdf

Định danh tư liệu:

DOI: https://doi.org/10.1186/s43141-023-00519-4 | ISSN 1687-157X

Nguồn gốc:

Journal of Genetic Engineering and Biotechnology, Volume 21, Issue 1, December 2023, 63

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