Analysis of catecholamines and related compounds in one whole metabolic pathway with high performance liquid chromatography based on derivatization

Loại tài liệu:

Article

Tác giả:

Huang, Xiao

Đề mục:

Arabian Journal of Chemistry

Nhà xuất bản:

Elsevier Ltd.

Ngày xuất bản:

November 2019

Định dạng:

pdf

Nguồn gốc:

Arabian Journal of Chemistry, Volume 12, Issue 7 , November 2019, Pages 1159-1167

Ngôn ngữ:

eng

Lượt xem:

Lượt tải:

Nội dung

The simultaneous determination of Tyr, catecholamines and their metabolites in one whole metabolic pathway using high performance liquid chromatography coupled with fluorescence detection (HPLC–FLD) based on pre-column derivatization has been achieved successfully. 1,3,5,7-Tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)-difluoroboradiaza-s-indacene (TMBB-Su), a highly reactive fluorescent reagent synthesized in our previous work, was used for the labeling of tyrosine (Tyr), l-3,4-dihydroxyphenylalanine (l-DOPA), dopamine (DA), norepinephrine (NE), epinephrine (E) and metanephrine (MN). Derivatization conditions including reagent concentration, buffer, reaction temperature and reaction time were also investigated to improve the derivatization efficiency and thus the sensitivity of the detection. The separation of the derivatives was obtained on C18 column with the mobile phase of 20 mM pH 3.5 citric acid (H3Cit)–sodium hydrogen phosphate (Na2HPO4) buffer and methanol. Good linearities with correlation coefficients square (R2) greater than 0.998 in the corresponding concentration ranges were observed and the detection limits (S/N = 3) were found in the range from 0.10 to 0.40 nM (l-DOPA: 1.45 nM). The proposed method has been applied to the detection of catecholamines and related compounds in mice liver and brain samples without tedious extraction or purification procedure, which exhibits excellent selectivity and sensitivity in the analysis of complex samples. This work provides an alternative approach in the metabolic research of catecholamines and is helpful for the study of catecholamine metabolism.

Tập tin đính kèm

Loại file Tên file Dung lượng Chi tiết
201907AJC1159-1167.pdf 538054 Kb Xem Tải

Analysis of catecholamines and related compounds in one whole metabolic pathway with high performance liquid chromatography based on derivatization

THƯ VIỆN ĐẠI HỌC KHOA HỌC HUẾ