Development a novel in silico mRNA vaccine against Clostridium difficile

Clostridium difficile, a toxin-producing bacterium, can lead to conditions ranging from mild diarrhea to severe pseudomembranous colitis. Our research introduces an innovative strategy for developing a multi-epitope mRNA vaccine using in-silico methods targeting proteins involved in Clostridium difficile infection (CDI). Through computational models, we identified epitopes for B cells, helper T lymphocytes (HTL), and cytotoxic T lymphocytes (CTL) from protein sequences, assessing their immune response potential, allergenicity, toxicity, and autoimmune risk. The constructed 3D model underwent interaction docking and immune simulations, including evaluations of population coverage and physicochemical properties. Finally, molecular dynamics simulations were employed to test the vaccine's stability. Our findings suggest that this approach holds promise for creating a multi-epitope mRNA vaccine against C. difficile infection.

Loại tài liệu:

Article - Bài báo

Tác giả:

Nasim Cheraghi

Đề mục:

Journal of Genetic Engineering and Biotechnology

Nhà xuất bản:

Elsevier

Ngày xuất bản:

September 2026

Số trang/ tờ:

Định dạng:

pdf

Định danh tư liệu:

DOI: https://doi.org/10.1016/j.jgeb.2026.100771 | ISSN 1687-157X

Nguồn gốc:

Journal of Genetic Engineering and Biotechnology, Volume 24, Issue 3, September 2026, 100771

Loại file Tập tin đính kèm Dung lượng Chi tiết
2026N3JGEB100771.pdf 10750164 Kb Xem Tải